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equipment laminar flow hood  (Labconco)


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    Structured Review

    Labconco equipment laminar flow hood
    Equipment Laminar Flow Hood, supplied by Labconco, used in various techniques. Bioz Stars score: 96/100, based on 401 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/equipment+laminar+flow+hood/Logic+Biological+Safety+Cabinet/pmc07864545-73-205-209
    Average 96 stars, based on 401 article reviews
    equipment laminar flow hood - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: Vero C1008 (clone E6, ATCC, #CRL-1586) icSARS-CoV-2 (infectious cloned virus, UTMB, ( Xie et al., 2020 )) Horseradish peroxidase Avidin D (Vector laboratories, Inc #A-2004) Biotin labelled anti-SARS-CoV spike antibody (CR3022, Emory University, ( Suthar et al., 2020 )) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, 353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Saponin (Sigma, 47036-250G-F) Bovine Serum Albumin (BSA) (VWR, #0332–100g) KPL TrueBlue Peroxidase Substrate (VWR, # 95059–168) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (Rainin LTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (−60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Human samples The plasma samples used for this study were collected at Emory University Hospital and Emory University Hospital Midtown in Atlanta.

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: Materials Vero C1008 (clone E6, ATCC, #CRL-1586) icSARS-CoV-2 (infectious cloned virus, UTMB, ( Xie et al., 2020 )) Horseradish peroxidase Avidin D (Vector laboratories, Inc #A-2004) Biotin labelled anti-SARS-CoV spike antibody (CR3022, Emory University, ( Suthar et al., 2020 )) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, 353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Saponin (Sigma, 47036-250G-F) Bovine Serum Albumin (BSA) (VWR, #0332–100g) KPL TrueBlue Peroxidase Substrate (VWR, # 95059–168) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (Rainin LTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (−60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Human samples The plasma samples used for this study were collected at Emory University Hospital and Emory University Hospital Midtown in Atlanta.

    Modification:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Saline:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Cell Culture:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Sterility:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Membrane:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Electron Microscopy:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Polymerase Chain Reaction:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Blocking Assay:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Microscopy:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Transferring:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..

    Variant Assay:

    Article Title: Development of a rapid Focus Reduction Neutralization Test Assay for measuring SARS-CoV-2 neutralizing antibodies
    Article Snippet: .. Additional Materials icSARS-CoV-2-mNG Cellstar μClear blackout tissue culture treated plate (USA Scientific, #655090) Vero C1008 (clone E6, ATCC, #CRL-1586) Dulbecco’s Modified Eagle Medium (VWR, #45000–304) Dulbecco’s Phosphate-Buffered Saline (DPBS) (VWR, #4500–436) Fetal Bovine serum (Biotechne) Non-essential amino acids (Fisher Scientific, #11140050) L-glutamine (VWR, 25005Cl) HEPES buffer (VWR, #4500–690) Sodium pyruvate (VWR, #45000–710) Antibiotics (VWR, #45000–616) Trypan blue (Beta South Technologies, #T8154) Trypsin-EDTA 0.25% (Thermo Fisher Scientific, #25200072) 96 well Flat bottom cell culture treated plate (Grenier Bio-One, #655180) Falcon 96 well U-bottom plate (Corning, #353077) Methylcellulose (Sigma, #M0512–250) Paraformaldehyde (PFA), 20% (Fisher Scientific, #47340–9M) Serological pipettes, sterile (VWR or equivalent) 15 mL conicals VWR (89039–664 or equivalent) 50 mL conicals (89039–664 or equivalent) Tips for Micropipettes, sterile (RaininLTS or equivalent) 1.5 mL RNA / DNase free tubes (VWR 10160–142 or equivalent) Vacuum filter system polyethersulfone (PES) membrane 0.22 μM, sterile (Genesee Scientific, #25–227) 20% Formaldehyde solution (Electron Microscopy Sciences, #15713-S) Hyclone Powder Tissue Culture Media (VWR, #1677–047) 96-well non-skirted PCR Plate or similar (Thermo Fisher Scientific, #AB0600) Equipment Laminar flow hood (Labconco Purifier BSC Class II, or equivalent) Heat block or Water Bath (set to 56°C) Tissue Culture Incubator (set to 37°C with 5% CO 2 ) Refrigerator (2–8°C) Freezer (–60°C to –90°C) Tissue Culture Microscope (Nikon or equivalent) Autoclave Multichannel Pipette (200 μl and 20 μl) Analytical scale Labnet Rocker 25 (Model S2025-XLD-B) Benchmark Scientific Everlast Rocker 247 Special Equipment ELI-SPOT reader (CTL Analyzers or equivalent) Note: For the alternate protocol a variant of SARS-CoV-2, icSARS-CoV-2-mNeonGreen is used instead of the WT icSARS-CoV-2. ..



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